ISSN 2146-8133
 

Original Research (Original Article) Open Access


Comparison of the acute effects of hemostatic agents on neural tissues in spine surgery: Histologic analysis in rat models

Gokhan Meric, Omer Akcali, Serap Cilaker Micili, Alper Bagriyanik.

Abstract
Objective: The most frightening and likely complication of chemical hemostatic agents is neurologic deficit. The histological basis of this potential complication is still unknown. The aim of this study is to observe the acute histologic effects of routinely used hemostatic agents on neural tissues in a rat model.
Methods: Eighteen Winstar Albino rats were operated and same-level laminectomies were performed. The rats were divided into three groups. In group 1 (control group), surgical layers were sutured in routine manner after laminectomy. In group 2 (gelatin sponge group), dura mater was covered with gelatin sponge after laminectomy, while oxidized cellulose was used for coverage in group 3 (oxidized cellulose group). Neurologic evaluations were made for all test subjects. Fortyeight hours after the operation, rats were sacrificed and lumber spines were excised with all surrounding tissues for evaluation by light microscopy of the acute effects of agents on neural tissues. Neurologic scores and histologic findings were compared with double-blind evaluation.
Results: There were no statistically significant differences between the three groups in the histologic findings and clinical evaluations. However, the inflammatory reaction was more severe in the oxidized cellulose group.
Conclusion: Both gelatin sponge and oxidized cellulose did not increase the cellular necrosis of neural tissues. However, oxidized cellulose may lead to an increased local inflammatory reaction.

Key words: Oxidized cellulose, absorbable gelatin sponge, hemostasis, spine surgery, rat model


 
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REFERENCES
1. Zheng F, Cammisa FP Jr, Sandhu HS, Girardi FP, Khan SN. Factors predicting hospital stay, operative time, blood loss, and transfusion in patients undergoing revision posterior lumbar spine decompression, fusion, and segmental instrumentation. Spine (Phila Pa 1976) 2002;27:818-24. [DOI via Crossref]   
2. Shapiro F, Sethna N. Blood loss in pediatric spine surgery. Eur Spine J 2004;13(Suppl 1):S6-17. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
3. Schonauer C, Tessitore E, Barbagallo G, Albanese V, Moraci A. The use of local agents: bone wax, gelatin, collagen, oxidized cellulose. Eur Spine J 2004;13(Suppl 1):S89-96. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
4. Sabel M, Stummer W. The use of local agents: Surgicel and Surgifoam. Eur Spine J 2004;13(Suppl 1):S97-101. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
5. Harris WH, Crothers OD, Moyen BJ, Bourne RB. Topical hemostatic agents for bone bleeding in humans. A quantitative comparison of gelatin paste, gelatin sponge plus bovine thrombin, and microfibrillar collagen. J Bone Joint Surg Am 1978; 60:454-6. [Pubmed]   
6. Arand AG, Sawaya R. Intraoperative chemical hemostasis in neurosurgery. Neurosurgery 1986;18: 223-33. [DOI via Crossref]   
7. Alander DH, Stauffer ES. Gelfoam-induced acute quadriparesis after cervical decompression and fusion. Spine (Phila Pa 1976) 1995;20:970-1. [DOI via Crossref]   
8. Brodbelt AR, Miles JB, Foy PM, Broome JC. Intraspinal oxidised cellulose (Surgicel) causing delayed paraplegia after thoracotomy-a report of three cases. Ann R Coll Surg Engl 2002;84:97-9. [Pubmed]    [PMC Free Fulltext]   
9. Henry MC, Tashjian DB, Kasowski H, Duncan C, Moss RL. Postoperative paraplegia secondary to the use of oxidized cellulose (Surgicel). J Pediatr Surg 2005;40:E9-11. [DOI via Crossref]    [Pubmed]   
10. Ereth MH, Schaff M, Ericson EF, Wetjen NM, Nuttall GA, Oliver WC Jr. Comparative safety and efficacy of topical hemostatic agents in a rat neurosurgical model. Neurosurgery 2008;63(4 Suppl 2):369-72. [DOI via Crossref]   
11. Awwad EE, Smith KR, Jr. MRI of marked dural sac compression by surgicel in the immediately postoperative period after uncomplicated lumbar laminectomy. J Comput Assist Tomogr 1999;23: 969-75. [DOI via Crossref]    [Pubmed]   
12. Nagamatsu M, Low PA. Oxidized cellulose causes focal neuropathy, possibly by a diffusible chemical mechanism. Acta Neuropathol 1995;90:282-6. [DOI via Crossref]    [Pubmed]   
13. Basso DM, Beattie MS, Bresnahan JC. Graded histological and locomotor outcomes after spinal cord contusion using the NYU weight-drop device versus transection. Exp Neurol 1996;139:244-56. [DOI via Crossref]    [Pubmed]   
14. Erstad BL. What is the evidence for using hemostatic agents in surgery? Eur Spine J 2004;13(Suppl 1):S28-33. [DOI via Crossref]    [Pubmed]    [PMC Free Fulltext]   
15. Tomizawa Y. Clinical benefits and risk analysis of topical hemostats: a review. J Artif Organs 2005;8:137-42. [DOI via Crossref]    [Pubmed]   
16. Arat YO, Dorotheo EU, Tang RA, Boniuk M, Schiffman JS. Compressive optic neuropathy after use of oxidized regenerated cellulose in orbital surgery: review of complications, prophylaxis, and treatment. Ophthalmology 2006;113:333-7. [DOI via Crossref]    [Pubmed]   
17. Nedergaard M, Goldman SA, Desai S, Pulsinelli WA. Acid-induced death in neurons and glia. J Neurosci 1991;11:2489-97. [Pubmed]   
18. Dodd CA, Fergusson CM, Freedman L, Houghton GR, Thomas D. Allograft versus autograft bone in scoliosis surgery. J Bone Joint Surg Br 1988;70: 431-4. [Pubmed]   

How to Cite this Article
Pubmed Style

Meric G, Akcali O, Micili SC, Bagriyanik A. Comparison of the acute effects of hemostatic agents on neural tissues in spine surgery: Histologic analysis in rat models. Arch Clin Exp Surg. 2016; 5(1): 21-26. doi:10.5455/aces.20150408072235


Web Style

Meric G, Akcali O, Micili SC, Bagriyanik A. Comparison of the acute effects of hemostatic agents on neural tissues in spine surgery: Histologic analysis in rat models. http://www.acesjournal.org/?mno=179999 [Access: January 19, 2018]. doi:10.5455/aces.20150408072235


AMA (American Medical Association) Style

Meric G, Akcali O, Micili SC, Bagriyanik A. Comparison of the acute effects of hemostatic agents on neural tissues in spine surgery: Histologic analysis in rat models. Arch Clin Exp Surg. 2016; 5(1): 21-26. doi:10.5455/aces.20150408072235



Vancouver/ICMJE Style

Meric G, Akcali O, Micili SC, Bagriyanik A. Comparison of the acute effects of hemostatic agents on neural tissues in spine surgery: Histologic analysis in rat models. Arch Clin Exp Surg. (2016), [cited January 19, 2018]; 5(1): 21-26. doi:10.5455/aces.20150408072235



Harvard Style

Meric, G., Akcali, O., Micili, S. C. & Bagriyanik, A. (2016) Comparison of the acute effects of hemostatic agents on neural tissues in spine surgery: Histologic analysis in rat models. Arch Clin Exp Surg, 5 (1), 21-26. doi:10.5455/aces.20150408072235



Turabian Style

Meric, Gokhan, Omer Akcali, Serap Cilaker Micili, and Alper Bagriyanik. 2016. Comparison of the acute effects of hemostatic agents on neural tissues in spine surgery: Histologic analysis in rat models. Archives of Clinical and Experimental Surgery (ACES), 5 (1), 21-26. doi:10.5455/aces.20150408072235



Chicago Style

Meric, Gokhan, Omer Akcali, Serap Cilaker Micili, and Alper Bagriyanik. "Comparison of the acute effects of hemostatic agents on neural tissues in spine surgery: Histologic analysis in rat models." Archives of Clinical and Experimental Surgery (ACES) 5 (2016), 21-26. doi:10.5455/aces.20150408072235



MLA (The Modern Language Association) Style

Meric, Gokhan, Omer Akcali, Serap Cilaker Micili, and Alper Bagriyanik. "Comparison of the acute effects of hemostatic agents on neural tissues in spine surgery: Histologic analysis in rat models." Archives of Clinical and Experimental Surgery (ACES) 5.1 (2016), 21-26. Print. doi:10.5455/aces.20150408072235



APA (American Psychological Association) Style

Meric, G., Akcali, O., Micili, S. C. & Bagriyanik, A. (2016) Comparison of the acute effects of hemostatic agents on neural tissues in spine surgery: Histologic analysis in rat models. Archives of Clinical and Experimental Surgery (ACES), 5 (1), 21-26. doi:10.5455/aces.20150408072235





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