ISSN 2146-8133
 

Original Research (Original Article) Open Access


Effects of bile on arterial anastomosis in a rat model

Kamil Kilic, Gokhan Temiz, Murat Zeytunlu, Murat Kilic, Mehmet Alper.

Cited by (1)

Abstract
Objective: Liver transplantation has been one of the areas in which microvascular surgery is frequently practiced. With the use of microsurgical techniques in hepatic artery anastomosis, the incidence of hepatic artery thrombosis has been signifi- cantly reduced. Hepatic artery anastomosis is realized after the anastomoses of the hepatic vessel and portal vessel are completed, and clamps are removed; in other words, after hepatic circulation starts. During hepatic artery anastomosis, bile runs towards the hepatic artery anastomosis line. This study aimed to investigate effects of bile on arterial anastomosis.
Materials and Methods: Forty male Sprague Dawley rats were randomly divided into 2 groups. Femoral artery anasto- mosis was performed in rats of the control group (n = 20) through heparinized serum (10 U/ml) irrigation. In the rats of the study group (n = 20), however, femoral artery anastomosis was performed through heparinized serum (10 U/ml)+0.5 cc bile irrigation. After the 1st week, anastomosis patency was evaluated with color duplex ultrasonography. A 1 cm segment of the femoral artery involving the anastomosis line was removed for histopathological evaluation.
Results: In the control group, no flow to the distal was observed in one anastomosis. All the anastomoses in the study group were patent. Histopathologically, perivascular polymorphonuclear leucocyte infiltration was observed in the study group. No statistically significant differences were observed for endothelial continuity and intimal thickening, and no statisti- cally significant differences were observed for anastomosis patency.
Conclusions: In the light of these findings, it was determined that bile contamination did not have any negative or positive effects on artery anastomosis.

Key words: Bile, hepatic artery anastomosis, microsurgery


 
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This Article Cited By the following articles

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Plastic and Reconstructive Surgery - Global Open 2015; 3(11): e570.

1
 

How to Cite this Article
Pubmed Style

Kilic K, Temiz G, Zeytunlu M, Kilic M, Alper M. Effects of bile on arterial anastomosis in a rat model. Arch Clin Exp Surg. 2015; 4(3): 135-141. doi:10.5455/aces.20141022045624


Web Style

Kilic K, Temiz G, Zeytunlu M, Kilic M, Alper M. Effects of bile on arterial anastomosis in a rat model. http://www.acesjournal.org/?mno=170494 [Access: January 19, 2018]. doi:10.5455/aces.20141022045624


AMA (American Medical Association) Style

Kilic K, Temiz G, Zeytunlu M, Kilic M, Alper M. Effects of bile on arterial anastomosis in a rat model. Arch Clin Exp Surg. 2015; 4(3): 135-141. doi:10.5455/aces.20141022045624



Vancouver/ICMJE Style

Kilic K, Temiz G, Zeytunlu M, Kilic M, Alper M. Effects of bile on arterial anastomosis in a rat model. Arch Clin Exp Surg. (2015), [cited January 19, 2018]; 4(3): 135-141. doi:10.5455/aces.20141022045624



Harvard Style

Kilic, K., Temiz, G., Zeytunlu, M., Kilic, M. & Alper, M. (2015) Effects of bile on arterial anastomosis in a rat model. Arch Clin Exp Surg, 4 (3), 135-141. doi:10.5455/aces.20141022045624



Turabian Style

Kilic, Kamil, Gokhan Temiz, Murat Zeytunlu, Murat Kilic, and Mehmet Alper. 2015. Effects of bile on arterial anastomosis in a rat model. Archives of Clinical and Experimental Surgery (ACES), 4 (3), 135-141. doi:10.5455/aces.20141022045624



Chicago Style

Kilic, Kamil, Gokhan Temiz, Murat Zeytunlu, Murat Kilic, and Mehmet Alper. "Effects of bile on arterial anastomosis in a rat model." Archives of Clinical and Experimental Surgery (ACES) 4 (2015), 135-141. doi:10.5455/aces.20141022045624



MLA (The Modern Language Association) Style

Kilic, Kamil, Gokhan Temiz, Murat Zeytunlu, Murat Kilic, and Mehmet Alper. "Effects of bile on arterial anastomosis in a rat model." Archives of Clinical and Experimental Surgery (ACES) 4.3 (2015), 135-141. Print. doi:10.5455/aces.20141022045624



APA (American Psychological Association) Style

Kilic, K., Temiz, G., Zeytunlu, M., Kilic, M. & Alper, M. (2015) Effects of bile on arterial anastomosis in a rat model. Archives of Clinical and Experimental Surgery (ACES), 4 (3), 135-141. doi:10.5455/aces.20141022045624





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